Molecular Study of Efflux MexX Gene in Pseudomonas aeruginosa Isolated from Iraqi Patients

  • Suhad H. Friyah , Marrib N. Rasheed

Abstract

Clinical isolates (95) collected from patients suffering different  infections from teaching hospital in Baghdad\ Iraq. These isolates cultured on specific media to grow Pseudomonas aeruginosa only . The growed  isolates (54) were diagnosed using classical methods  and the API 20E followed by molecular detection using a housekeeping gene (rpsL). Fifty four isolates of Pseudomonas aeruginosa tested against gentamicin. These isolates were selected to determine resistance mediated by MexX efflux system using polymerase chain reaction (PCR) . Out of 54 P.aeruginosa isolates, 48(88.8%) gave positive results for efflux system MexX (resistance and intermediate isolates), while  sensitive isolates 6 (11.2 % ) have no MexX gene .  This may indicate the prevalence these type of resistance in the current isolated bacteria.

 

Published
2019-02-02