Pancreatic Protective role of resveratrol extracted from peanut skin under Toxic Influence of Alloxan in Mice

Abstract

Oxidative stress plays an important role in the pathogenesis of a wide range of diseases, many of the plant chemicals (phytochemicals) in the foods are antioxidants. These nutrients the decrease free radicals and may reduce the damage they would cause in the body. Aim:  The objective of this study is to assess the activity of resveratrol derived from peanuts (Arachis hypogaea L.) in male albino mice given alloxan to create a diabetic model with pancreatic damage and elevated blood glucose. Methods: raw peanut pods were gathered from Iraqi markets, and then the Soxhlet equipment was used to prepare a 95% ethanol extract of resveratrol from peanut skin using reflux extraction. Numerous studies were carried out, such as biochemical assays and total phenolic content. By measuring blood glucose levels, the protective effects of resveratrol extract against Alloxan toxicity were assessed. Thirty mice were randomly assigned to five groups, each including six animals. Mice included in the experiment were given an alloxan injection on day one and were given resveratrol extracts at doses of 50 and 100 mg/kg for 42 days. Results: Treatment with resveratrol extract 50 mg/kg resulted in a significant decrease in serum glucose concentrations (126 mg/dl). The greatest effect was observed with resveratrol extract 100 mg/kg (111.67 mg/dl), which also showed a significant decrease when compared to the control group. Additionally, pancreatic tissue from animals given 150 mg/kg of Alloxan treatment exhibited histological deformation of the outline structure together with scattered necrotic endocrine cells. On the other hand, endocrine cell therapy with a 100 mg/kg resveratrol extract exhibited almost normal histological structure appearance. Conclusion: According to this study, resveratrol extract helped to decrease blood glucose levels in diabetic mice

Keywords: Alloxan, Pancreatic, resveratrol, Arachis hypogaea, Diabetic, Total phenol.