InVitro: Detection TaSOS1 gene in four Iraq genotypes of Bread Wheat under different salt stress levels.

Abstract

In this study, Four Iraqi genotypes of Bread Wheat (Iraq, 2H,3H and Hussein) were used to detection (TaSOS1) gene (a transmembrane Na⁺/H⁺ antiporter) under salt stress with different concentrations of NaCl (4,8,12 and 16 ds.m^-1) by using PCR technique. The results showed that Iraq cultivar was sensitive to salinity, It gave less values in all trait studies. 2H and Hussein genotypes were salt tolerance, It showed in most trait studies (Callus relative growth , Calli water content , Proliferation efficiency of dry weight, Regeneration frequency , shoot length  and K+/Na+ ) , 3H genotypes was salt tolerance but less degree from 2H and Hussein. The detection on gene by using PCR technique , Gene was shown in three genotypes (2H,3H and Hussein) but it was not found in Iraq . So this gene was found important to salt tolerance .