Allele Frequency of 15 Autosomal Short Tandem Repeat (STR) Loci in Al Anbar - Iraqi Population

Abstract

Allele frequencies for 15 STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317,D16S539, D18S51, D21S11, FGA, TH01, TPOX, VWA, D2S1338, D19S433) included in the AmpFlSTR Identifiler kit were determined in a sample of 132 unrelated people originating From Al Anbar - Iraqi Population, samples were extracted using a Prep Filer Forensic DNA Extraction Kit.

DNA concentration measured using Nano drop Simultaneous amplifications of 15 STR loci and a gender determination marker (multiplexed PCR) were done by using the AmpFlSTR® Identifiler® PCR Amplification Kit according to the user’s manual recommendations .

The separation and detection of amplified products were conducted with the 3130 xl Genetic Analyzer 16-capillary array system following manufacturer’s protocols, A different number of alleles were observed with frequencies ranging between 0.0038 (FGA- allele 18, 19.2, 23.2, 27 and 28 - , D18S51 - allele 20 and 23, D19S433 - allele 9.2,11 and 12.2, D2S1338 – allele 27, D13S317 – allele 16, D3S1358 – allele 13 and 19 CSF1PO – allele 14, D21S11– allele 30.2 and 32 and D8S1179 - allele 9 and 17) and 0.5795 (TPOX-allele 8). The highest heterozygosity is observed for FGA (85.60 %) where the smallest heterozygosity value is obtained for TPOX (61.36 %). The loci were observed to have high discriminating power, as the power of discrimination of each loci varied from 0.812 (TPOX) to 0.973 (D2S1338). All loci but D21S11 (<0.001), D19S433 (<0.001), D5S818 0.00204 and FGA (<0.00024) met Hardy- Weinberg expectations (P > 0.05). Significant departure from Hardy Weinberg Equilibrium (HWE) expectations were observed in loci D21S11 (0.001), D19S433 (0.001), D5S818 (0.002) and FGA (0.00).

The results of the population genetics tests and pairwise comparisons suggest that these allele frequency databases are suitable for the purpose of identification in paternity or forensic investigations.