Detection of MDR Gene (IFITM3) and P- glycoprotein Expression in Patients with Hodgkin's Lymphoma in AL-Ramadi Teaching Hospital

Abstract

The  present    study  aimed  to  shed light   on   the  follow  up  of  Hodgkin's   lymphoma  patients at  initial    diagnosis  and   after   treatment,   to  assess   the  response    and  early  relapse    after   chemotherapy  through   evaluating the  gene   expression level  of  one  of  the major   multidrug   resistance  genes which   is  the  multidrug   resistance 1 (MDR1),and  investigate  the   presence  of P-glycoprotein  by immunohistochemistry assay. The study was conducted on fifty Hodgkin's lymphoma patients during the period extended from January 2015 to November 2015. The patients included 41(82%) males and 9 (18%) females ,with ages ranged from 12 to 81 years. The study also included ten persons (7 males and 3 females ) as healthy control group . All patients were followed up by doing some laboratory test such as complete blood count before and after chemotherapy, and the tumor markers which  indicate the response  degree of   patients to chemotherapy

DNA was extracted from paraffin embedded tissue, which were   collected   from AL-Ramadi teaching Hospital / Histopathology unit, in order to detect the Interferon-induced trans  membrane protein  IFITM3 (MDR) gene using PCR technique .The results showed in the healthy individuals, there were no mutation presence in the  MDR gene in patients and control. 

Results of immunohistochemistry study showed that the presence of (MDR1) P glycoprotein   had    played a   great   role in regulation of the cancer cells growth. Depending on the intensity of coloration,  and  percent of cancer cells stained , the results indicated the production of P-glycoprotein in 15 (30%) case as 1+, 6 (12%) case as 2+ and 10 (20%) case as 3+,  while   other  cases  19 (38%) were negative.