Relationship Determination between Heart Diseases with Endometrial Adenocarcinoma and Ovarian Carcinoma by Evaluating the HPV16 IgG , IgM and p53 in Some Iraqi Female Patients

Abstract

Heart diseases problems linked to female genitalia associated carcinomas were considered as a major issues and life threat; therefore, it was necessary to determine the relationship between heart diseases problems with HPV16 infection and P53 expression in some groups of female Iraqi patients that suffer from endometrial Adenocarcinoma (EC) and ovarian carcinoma. This study was designed as a two prospective groups. It involved  ninety samples of serum were collected from the Medical City of Baghdad from the period of October 2014 to January 2015 as 45 \ 50% of endometrial Adenocarcinoma (EC) patients group and 45 (50%) of ovarian carcinoma patients group then stored in -80°C. All samples were examined for HPV16 IgG, IgM and p53 expression by the enzyme-linked immunosorbent assay (ELISA). Highly significant difference (p <0.001) of Mutant p53 expression has been detected in 32 \ 35.6% out of 41 \ 45.6% patients with heart diseases history in comparing with patients without history of heart diseases. In addition to , highly significant differences (p < 0.001) of HPV16 IgM has been detected  in 36 patients had previous history of heart problems as 40% out of 41 patients. In the other hand, Endometrial Adenocarcinoma (EC) cases, have been a statistically significant differences with HPV16 IgG in 35 \ 38.9 % and HPV16 IgM in 31 \ 34.4%  positive results out of 45 cases (p < 0.05 ) with highly significant differences in the positive cases of 32 \ 35.6% that had been detected with heart diseases problems (p < 0.001). Furthermore, the ovarian carcinoma cases have positives HPV16 IgG and HPV16 IgM levels as 24 \ 26.7 % and 18 \ 20% respectively with significant difference regarding to the negative results (p < 0.05 ). An interesting results has been detected recently in this study, as HPV16 infection followed by p53 inactivation may initiate heart diseases in some groups of females Iraqi patients that had been with endometrial Adenocarcinoma or ovarian carcinomas. So that, HPV16 infection plays a major role in the heart diseases progression through it E6 and E7 oncoproteins to the sabotage of  the normal p53 and pRb pathways.