Virulence Genes Profile of Pseudomonas aeruginosa Local Isolates from Burns and Wounds

Abstract

In this study  a total of 111 swabs were collected  from  50 patients suffering from burns and wounds  between the age   9 months -69 years  from both gender , the specimens  were collected from Educational Al-Yarmouk and AL Kadhimiya hospitals in Baghdad during the period between October-December\2014,  All the samples  were identified by biochemical tests , API  20 E and  Vitak -2   . From 111 specimens, 31 isolates  were Pseudomonas aeruginosa ,  33  isolates were Escherichia coli and 10 isolates were Klebsiella spp,  . Drug susceptibility tests for the 31 isolates of Pseudomonas aeruginosa were studied by the disk diffusion method against 5 antibiotics and the result showed the percentage of resistance: Carbenicillin  100%, Ticarcillin  61 %, Pipracillin 32 % ,Cefprozil 93% , Colistin 84%  . The  DNA samples of the  31 isolates of  Pseudomonas.aeruginosa were extracted by DNA extraction kit  ,  the concentration for  all  31  DNA samples  were between  60- 110  ng /ul  and  the purity were between 1.8-2  .PCR (Polymerase Chain Reactin) was used for  screening  the virulence factor genes , protein synthesis inhibition  genes (exo A  , las B , exoU ) of the  thirty one  isolates of  Pseudomonas  aeruginosa . The result showed that  27  ( 87%) isolates  were positive for exo A and las B genes, 4            ( 13%) were negative, , from 25 burn isolates  was 23 (92%) positive isolates and  from 6 wound isolates was 4 (66%) positives  isolates.  . For the exoU gene the result showed that  17  ( 55%) were positive , 14 ( 45% )were negative , From 25 burn isolates  the presence of exoU was seen in 15 (  60 %), while from 6 wound  isolates was 2 (33 %).