Molecular detection of Proteus mirabilis using PCR technique among urinary tract infection patients

Abstract

The study included isolation and diagnosis of bacteria Proteus mirabilis from patients suffering from urinary tract infection (UTI) with different types (simple and complicated cases) and   from clinical urinary tract infected patients. Isolated of bacteria from (UTI) infected patients and from patient urinary tract catheter which was used in complicated cases and from defective urinary tract patients. Three hundred and ten (310) samples from (UTI) patients in different hospitals of Baghdad and Al-Kut.  From period 1/1/2013 to 1/8/2013.

The results observe (218) positive isolates for bacteriological exam and by the biochemical tests and Api appear Proteus spp (17.88 %) and P. mirabilis  ( 92.3%) but P. vulgaris(7.7) %.

 PCR by used specific primer (16SrRNA) for detected genus Proteus and to differentiate it from other enterobacteria used (UreR) primer to detect the genome which was controlled for urease enzyme production. The results of primer UreR observe 36 isolates were positive.

 Results of the Nitrogen bases sequence of the PCR technology of the samples in this study revealed consistency reaching up to 98 % with the Nitrogen bases sequence of the UreR gene present in the P. mirabilis strain of the WHO. This study presented high specificity and sensitivity for the diagnosis of  P. mirabilis using the PCR technology which is cheaper and faster than the conventional methods currently used in the hospitals and laboratories.