hsp70-2 Polymorphism in Urinary Bladder Carcinoma

Abstract

hsp70-2 or (hspa1b) is one of the human HSP70 genes, it was originally found specifically in primary spermatocytes and spermatids, that played an important role in spermatogenesis. Now HSP70-2  has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. HSP70-2 has been identified as a potential cancer-promoting protein expressed at abnormal levels in a subset of human cancers such as bladder urothelial cancer. So, the present study aimed to study  the HSP70-2 polymorphism   in 50 blood sample of patients with urinary bladder carcinoma (UBC) and 15 healthy subjects, also study  HSP70-2 gene expression in 40 paraffin tissue sections of  patients with urinary bladder carcinoma UBC and 10 paraffin tissue section in patients with urinary bladder diseases UBD. A PCR-RFLP test was used to study the HSP70-2 gene polymorphism. r RT-PCR was used to study HSP70-2 gene expression. Genotype distribution and allele frequencies of HSP70-2 polymorphisms showed HSP70-2 P1P2 genotype frequency and HSP70-2 P2 allele were  significantly increased  in the  patients with UBC compare to healthy subjects(p≤0.01).Also, the  expression  mean of  HSP70-2 gene is 39.4 fold higher in UBC  than  in UBD patients.