Detection of K-ras Codons (12\13 and 61) Genetic Variations in Iraqi

Abstract

Breast cancer is the most common type of malignancy recorded in the cancer registries of almost all countries within the Eastern Mediterranean Region and most prevalent malignancy in women in Western countries currently accounting for one third of all female cancers. K-ras is proto-oncogene is a Kirsten ras oncogene, K-ras, is a protein that in humans is encoded by the K-ras gene located on the short arm of chromosome 12 (12p12.1). Women who are not carriers of BRCA genes may have a K-ras-variant. Paraffin-Embedded (FFPE) were used to detection of the K-ras genetic aberrations in breast cancer Iraqi patients and their consent were taken, to determine K-ras gene ability to predict prognosis, by extracting the DNA from the samples and amplified the area of interest by using the modern techniques for genetic analysis like HRM (High resolution melting) and Sequencing by using specific designed primers. The aim of this study to assessments of K-ras patients with breast cancer and find the correlation between the K-ras mutations and breast cancer. In this study K-ras gene mutations can be present in breast cancer patients representing somatic mutations. However, they should be confirmed as a germ-line mutation in patients. I recommend establishing K-ras mutations in breast cancer patients as a routine study in specialized centres by amplification and sequencing and detection more variation especially in BRCA