Study the effects of STRA 6 gene polymorphism on the incidence of T2DM in a sample of Iraqi patients

Abstract

This study was aimed to detect STRA6  gene  polymorphism in Iraqi type 2  diabetes mellitus patients (T2DM) to found the correlation between the SNP (rs 736118) polymorphism  in  STRA6  gene  and lipid metabolism and impact on the incidence  of  type 2  diabetes mellitus (T2DM) . Genomic DNA was extracted from the blood samples of T2DM patients and apparently healthy as a control group by using genomic DNA purification kits (Geneaid), the purity of the DNA was between   1.7 -1.9 and the concentration between 30 -100 ng / μl,. total cholesterol TC , high density lipids HDL, low density lipids LDL and Triglycride and fasting blood sugar FBS were measured by kits supplied by (Biolabo, France), while glycosylated hemoglobin HbA1C  measured by kit supplied by (Nycocard, Norway). Mean value of triglycerides in diabetic patients was significantly (p<0.001). The result show high Significant difference was observed in FBS level  (p<0.001)  in patient group (183.47 ± 6.32) and  in control  (88.61 ± 3.70), in other hand high Significant difference was observed in HBA1C level was   (p<0.001)  in patient group ( 8.87 ± 0.21 ), while in control group  was (4.86 ± 0.10). Cholesterol mean level value in diabetic patients was significantly higher than those of control group  (p<0.001) , LDL-Cholesterol mean value in diabetic patients was statistically significant (p<0.005) higher than the mean value of control group, (p<0.001), Serum HDL-Cholesterol mean value was significantly (p<0.001), VLDL-Cholesterol mean value in diabetic patients was significantly (p<0.001) increased compared to the  mean of control group. Real time PCR (Taq man ) RT-PCR  were used to detect  SNP (rs 736118) C>T  in STRA6 gene by using specific probes and  primers,   As a related  with  SNP (rs 736118) C>T  in STRA6 gene , the percentage of those CT polymorphism genotype in the patients  group was significant  (p<0.001)   compare with  control group (58% versus 0% respectively) and the individuals with the CT genotype in the patients group is significantly  higher  than those with  CC  genotype, the percentage of those with TT polymorphism genotype  in the patients   group is significantly higher as compared with control group (6% versus 0% respectively . The percentage of those with CC polymorphism genotype  in the patients   group show highly significant differences as a compared with control healthy group (28%, 0% respectively), as a conclusion of this study there was a relationship between polymorphism of STRA 6 gene SNP (rs736118) and the incidence of T2DM in sample of   Iraqi patients.