A Study of FLT/ITD Mutations in Cytogenetically Normal Iraqi Acute Myeloid Leukemia Patients

Abstract

The present study was designed to shed light on the molecular effects caused by acute myeloid leukemia (AML) pathogenesis in three cases before, during and after treatment with chemotherapy (in vitro) in lymphocytes. It was also aimed to investigate FLT3/ITD point mutations in cytogenetically normal-AML region 100-300 bp compared to healthy control. The study comprised of 30 AML Iraqi patients and their ages ranged between 2.5-81 years. It included 12 females and 18 males compared with 26 healthy controls. Results revealed that the extracted DNA from 30 AML patients and amplified by PCR to obtain FLT3/ITD gene from exon 11 to 12 showed larger bands (470 and 460) bp in 2 patients when compared to wild type (330) bp. Among six patients, three of them displayed point mutations of deletion and substitution, while the others were normal since no mutations were detected. The percentages of mutation types were substitution 77.8% and deletion 22.2%. The types of mutations were missense mutations as (55.54%), deletion (22.22%) and nonsense (22.22%).