Evaluation of Oncogene Protein p190/bcr-abl in some Iraqi chronic myelogenous leukemia patients

Abstract

Philadelphia chromosome is the most common specific cytogenetic chromosomal abnormality in human leukemias that is associated with chronic myelogenous leukemia (CML). However, the presence of the Philadelphia (Ph) chromosome is not sufficiently specific to diagnose CML, it is also found in acute lymphoblastic leukemia (ALL) and occasionally in acute myelogenous leukemia (AML). The purpose of this study was to use an enzyme-linked immunosorbent assay (ELISA) to detect Philadelphia chromosome in small samples of cells for ALL patients. Sera from 50 ALL Iraqi patients were used for evaluation of Oncogene Protein p190/bcr-abl. Also, Sera from a total of 38 healthy humans were used as negative controls to determine the referral intervals value for ELISA. While the range of oncogene protein p190 / bcr-abl concentration value for healthy control group was 0.111-0.161 ng/ml, the upper limit considered as a referral interval value of the 99% confidence interval (CI). However, ELISA values with concentrations above the assay referral interval of  0.161 ng/ml revealed Ph+ALL. The sera of 9 out of 50 ALL Iraqi patients showed a significantly higher levels than the referral interval (p>0.01).