Screening of Production Pyocin S5 Isolate from Multidrug Resistant Pseudomonas aeruginosa

Abstract

This study included collecting (120) clinical specimen from different sources, (43) isolates successfully were diagnosed as P. aeruginosa, representing 35.8% of total isolates. The test for the antibiotics susceptibility of the bacteria to (10) types of antibiotics showed a clear variation in their resistance to antibiotics, as all isolates showed resistance (100%) against to cephalexin, (90 %) against to Gentamicin and 82% against to Ceftazidime,14 isolate out of 43 isolate had MDR. While the results of the phenotypic detection of pyocin production to resistance isolate (14 isolate) showed the presence of 12 isolates (85.7%), The third screening stage molecular detection of the pyocin S5 gene, which is one of responsible for the production of Pyocin S5, It was shown that 6 isolates (50%) possess this gene, while 6 isolates (50%), they did not possess this gene.