Production, Purification and Biochemical Characterization of Lipase from Pseudomonas aeruginosa

Abstract

: Lipases are enzymes that have numerous applications in many industries like leather, soaps and detergents, pharmaceuticals, biofuel, food, textile, etc. Many organisms like plants, fungi, bacteria are known to produce lipase. The present study was aimed at isolating lipase producing microorganisms from different water samples which are rich in lipid content like oil mills. As oil mill samples are rich in lipid and fatty acid content it makes a very good source to find microorganisms capable of degrading lipids by producing lipase enzymes. In the present study the isolates were identified by morphological, biochemical and molecular characterization. Purification results of lipase exposed prominent specific activity of 248.4 U/mg, purification fold of 75.50, and 47.7% yield. The purified lipase demonstrated outstanding activity and stability in a temperature range of 25-45 °C and pH (5–10), revealing optimal activity at 35 °C and pH 7. The molecular weight of the enzyme was estimated to be 63 kDa. Compared to control, the lipase activity was promoted in the presence of calcium chloride.