Lack of Association Between PTPN22 1858 C>T Gene Polymorphism and Susceptibility to Generalized Vitiligo in a Iraqi Population

Abstract

The protein tyrosine phosphatase nonreceptor 22 (PTPN22) is associated with susceptibility to group of autoimmune diseases. The functional polymorphism in PTPN22 at 1857 It is considered  a risk factor for vitiligo susceptibility in European;  however, controversy exits in other populations. Present study aimed to study The exon 14 region of the PTPN22,and determine whether the PTPN22 C1857T polymorphism confers susceptibility to vitiligo in Iraqi patients. Genomic DNA was extracted and amplified using   polymerase chain reaction and sanger sequencing method. The frequency of the CC and CT genotypes were 97.30% (36) and 2.70% (1) in the generalized vitiligo patients, respectively, and 93.88% (46) and 6.12% (3) in the healthy controls, respectively. There was no statistically significant difference between the generalized vitiligo patients and healthy controls.The homozygotes genotype (TT) was absent in both the generalized vitiligo patients and healthy controls. there was no significant difference between two groups. The frequencies of the polymorphic T allele were 1.28% and 3.06% in the patient and the control groups. The genotype and allele frequency of the rs2476601 did not show any statistical significance (p > 0.05) in its association with generalized vitiligo.