The Effect of Genetic Polymorphism of Resistin Gene among Iraqi Breast Cancer Women

Abstract

 Breast cancer is a heterogeneous disease defined by molecular types and subtypes. It constitutes the most commonly-diagnosed cancer and the leading cause of cancer death in women, worldwide, according to the International Agency for Research on Cancer (IARC) World Cancer Reports in 2020. The study aimed to detect the relationship between Resistin Gene polymorphism (rs1862513, rs3745367) with the age and BMI of the participants diagnosed with breast cancer.  A total number of 105 samples; three groups of 35 fresh blood samples were collected as malignant, benign and apparently healthy control. Conventional PCR with specific primers followed by sanger sequencing was used to characterize the two SNPs. The results revealed that (rs1862513) GG allele carriers (GG) had a breast cancer risk of (1.78) in compare with (CG) allele carriers; (OR=0.337). Genotyping and allele frequency of RETN gene at SNP (rs3745367) in three groups (control, benign and malignant) showed that AA allele with (OR=1.44) may considered as a risk factor for incidence of breast cancer. The results showed that the incidence of breast cancer was significantly higher in middle age group (40-49) year with a percentage of 42.85%. It was concluded the relation of BMI and breast cancer remains conflicting, the study show a negative correlation of BMI with tumor size, stage and grade of the tumor.