Investigation of IL-23, IL-17 and Calprotectin Levels in Blood of Iraqi Patients with Ulcerative Colitis

Abstract

Ulcerative colitis (UC) is a type of inflammatory disease of the colon and it causes heavy inflammation. Knowing about cytokines is very important due to their role in the progression of the disease and in decreasing its severity. The current study aims to estimate calprotectin, IL-17, and IL-23 in the blood of Iraqi patients with ulcerative colitis. Also measuring levels of inflammation markers including ESR, WBCs, and CRP, and the reason for its height in serum or blood of ulcerative colitis patients. In this study, 30 blood samples from Iraqi patients with UC and 30 blood samples from Iraqi healthy individuals as control have been collected from 2021/11 to 2022/4, the samples were collected from Medical City Hospitals, Gastro-Enterology and Hepatology Hospital in Baghdad, Iraq. Calprotectin, IL-23, and IL-17 were measured by the enzyme-linked immune-Sorbent assay technology (sandwich-ELISA method). The Biomarkers used in clinical practice to assess disease activity are complete blood count (CBC), c-reactive protein (CRP), and erythrocyte sedimentation rate (ESR).  The current study results are the incidence of ulcerative colitis between males and females is not statically significant, the mean age of diagnostic of ulcerative colitis is 33.93 ±2.59, and it has been noticed a high incidence of ulcerative colitis in Iraqi people who have medium economic status. It was concluded significant differences in levels of calprotectin, IL-17, and IL-23 compared to the control group. Also, higher levels of ESR, WBCs, and CRP in ulcerative colitis compared to the control group.