Antibacterial Activity of Chitosan Nanoparticles Loaded with Syzygium aromaticum Extract against Klebsiella pneumonia

Abstract

The present study aims to describe the antibacterial activity of chitosan nanoparticles loaded with Syzygium aromaticum extract against MDR Klebsiella pneumonia. 50 isolates of K. pneumoniae were collected from patients with urinary tract infections. The isolates were diagnosed as Klebsiella pneumoniae by classical biochemical tests and PCR technique using 16S rRNA gene as a diagnostic gene and gel electrophoresis. The sensitivity of bacteria to antibiotics was tested using the Kerby-Bauer method. Measuring the diameters of inhibition and testing the effectiveness of the chitosan nanoparticles prepared in a previous study using spared plate method and counting the colonies growth. It was concluded to demonstrate that the chitosan nanoparticles loaded with Syzygium aromaticum extract have good antibacterial efficacy against MDR Klebsiella pneumonia.