Role of IFIH1 Gene Polymorphism rs3747517 and IL-17A with its Receptor in Detection and Therapeutic approach for Type 1 Diabetes Patients

Abstract

Type 1 diabetes (T1D) is multifactorial autoimmune disease, overlapping factors including genetic and environmental factors contribute to the appearance of T1D in children mostly and adults. The aim of the study was to find the correlation of T1D with the chosen immune parameters and with IFIH1C polymorphisms as genetic factor for the disease and study the role of interleukin-17 in the exacerbation and evolution of T1D. This study at pediatric Teaching Hospital in the medical city between November 2021 and January 2022.The study included 100 Iraqi children divided into two groups 50 T1D patients and 50 controls. Two SNPs (rs3747517 and rs1990760) from the IFIH1 gene were carefully chosen to examine their relationship with T1D. Human IL-17A and IL-17AR enzyme linked immunosorbent assay (ELISA) kits were used to estimate the levels of interleukins mentioned above. The recognition of SNPs was achieved by using HRM (high resolution melting) real-time PCR. A Rotor gene was employed to perform qPCR-HRM, followed by an HRM analysis with ramping by 0.2 °C from 55 to 95 °C. In both selected parameters  there was significant differences with average (IL-17A=260.0 , IL-17RA=238.4) of patients and an average (IL-17A=93.0, IL-17RA=90.9) in control group the p -value (0.001)  ,  data of IFIH1 gene polymorphism rs3747517  presented as shown in wild TT, hetero TC and mutant CC genotype ,comparison  between IL-17A and IL-17RA and TT,TC,CC genotype in patients group result no significant value for the IL-17A( p =0.106) but there was   a significant consequence between IL-17RA and SNP data (p =0.018). The IL-17A and IL-17R levels is a key indicator of damaged β cell function and progression to explicit T1D with it is receptor. It was concluded that IL-17A involvement in T1D increasing and a possible therapeutic approach for T1D directing IL-17A with IL-17RA besides highlight on status of IFIH1 as significant genetic factor and indicator to T1D.