Antibacterial and Cytotoxic Effect of Synthesized CuoNPs from Staphylococcus epidermidis

Abstract

The risky of major concern resistance antibiotics become for public health. The alternative treatment metallic nanoparticles such heavy metals effects on antibiotic resistance bacteria different type antibiotic- impossible to treat using novel eco-friendly synthesis technique nanoparticles. The aim of the study to search for alternative antimicrobial medicines has been prompted by growing worries about multidrug resistance bacteria by synthetize novel nanoparticles. This study was to explore whether green synthesized copper nanoparticles (INPs) by multi drug resistance bacteria Staphylococcus epidermidis isolation from wound and burn infection.  The results showed remarkable antimicrobial activity against Staphylococcus. aureus Minimum inhibitory Concentration range (16, 32, 64,128, 256, 512) µg/ml via well diffusion method in vitro, discover those concentrations effected in those bacteria and the best concentration is 64 µg/ml, characterization CuO NPs to prove this included atomic force microscope, Ultraviolet-visible spectrophotometer, X-ray Diffraction and Transmitted electrons microscopy, field emission scanning electron microscopy   and anticancer activity was tested against cell membrane A375. The cells viability was decreased with increasing the copper nanoparticles and displayed a dose-dependent sequence of progressive cytotoxicity beginning at lower concentration to its maximum inhibition, (22) % inhibition human dermal fibroblast neonatal (HdFn) cells and (66) % inhibition of A375cells. It was concluded CuONPs were successfully synthesized friendly environment, few cost, highly CuO NPs product showed exceptional activity against bacterial strains and activity against cell line A375cells invivo.