Molecular Study of Siderophore Genes in Carbapenems Resistant Klebsiella pneumoniae Isolated from Urinary Tract Infection Patients

Abstract

Urinary tract infections (UTIs) frequently result from gram-negative, bacillus bacteria one of them is called Klebsiella pneumoniae .These pathogens are capable of acquiring genes that code for a variety of antimicrobial resistance mechanisms, such as carbapenem resistance. The goal of the presented work was to identify siderophore genes in carbapenemase K. pneumoniae bacterial isolates from urine samples collected from patients with complicated UTIs at five hospitals in Baghdad, Iraq. From November 2022 until the end of February 2023, samples have been collected,from five hospitals in Baghdad.A total of 180 urine samples from outpatients and inpatients who attended were taken In contrast to the ratio of male patients (38.33%), the majority of patients (61.67%) were female. In sterile containers, midstream urine (MSU) samples have been taken from patients exhibiting UTI symptoms. The samples have been incubated at of 37° C for 24 hrs while being grown on MacConkey agar, blood agar, and Hi Chrome UTI Agar. Through watching grown colonies and isolating Gram-negative bacteria, the outcomes were documented. Out of (180) clinical samples ,n=50 (27.77 %) isolates  were  recognized as K.pneumoniae ,then selected (10) resistance isolates to made the detdction of entB and ybtS genes  .In this study, all n=10 (100%)  isolates were determined positive for 16srRNA housekeeping gene,  n = 10 (100%) of isolates were determined positive for the ybtS gene, and  n = 9 (90%) of isolates were positive for entB gene. The current study was concluded, the high virulence factor regarding carbapenems CRKP could be due to large part to high rate of the detection of ybtS and entB genes in highly virulent CRKP.