Synergistic Effect of Staphyloxanthin and some Antibiotics on Clinical Isolates of Klebsiella pneumoniae

Abstract

Klebsiella pneumoniae is one of the main causes of UTIs around the world. The aim of the study to examine the impact of staphyloxanthin and combination with some antibiotics on K. pneumoniae.150 urine samples obtained from Baghdad hospitals. The collected samples were streaked onto macConkey agar, followed by biochemical tests; then, using VITEK-2-compact system; thirty isolates were confirmed as K. pneumoniae. In addition, 10 pre-isolated and identified Staphylococcus aureus (producers of staphyloxanthin) were taken. The staphyloxanthin pigment extracted, purified. Antibiotic susceptibility testing of (30) K. pneumoniae isolate was assessed using KB testing. MIC of antibiotics (meropenem and ciprofloxacin) and staphyloxanthin were assessed for isolates using broth microdilution method, the effect of combination of staphyloxanthin and antibiotics was also evaluated for the same tested isolates. The result exposed that Meropenem MIC was 2 µg/ml, while MIC for staphyloxanthin pigment was 250 mg/ml. Ciprofloxacin MIC was 1000 µg/ml regarding the synergistic effect of the (ciprofloxacin and meropenem) combined with the Staphyloxanthin their MIC decreased for all, the results showed. It was concluded staphyloxanthin and antibiotics (ciprofloxacin and meropenem) have impact on K. pneumoniae.