ThiopurineMethyl Transferase (TPMT) Gene Expres-sion in Imuran and Biological Response in Ulcerative Colitis in Iraqi Patients

Abstract

Ulcerative colitis (UC) is a chronic IBD that involves the rectum and colonic mucosa. The study aim to expression in biological therapy more than its expression in Imuran treatment.This study included 50 patients with UC and 22 healthy. The RNA was extracted by the triazole method and the ages ranged from 15-50 years. The response toImuran treatment was weak, so they were referred to biological therapy. The gene expression of 22 patients was lower than the control ratio, meaning low gene expression, and the folding percentage was over gene expression.Venous blood samples were collected and preserved in TRIzol tubes, RNA extraction was performed, cDNA was synthesized, PCR was performed, expression levels were calculated and T-tests used to measure gene expression.Thiopurines are immunomodulators used for UC, and TPMT gene expression see significant value in Iraqi patients with high expression in biological therapy more than its expression in Imuran treatment groups.It was concluded gene expression of TPMT have a.significanteffect in Iraqi patients with UC.