Impact of Gene Polymorphisms (Rs5046) and Serum Levels of AGT Gene Attribution in Prevalence of Hypertension Patients

Abstract

Some genes might probably contribute to the regulation of blood pressure due to their previously established effect on cardiovascular or renal function, in addition to physical and environmental factors. The aim of the study detects the relationship between the genetic polymorphism of AGT and the level of AGT in serum and its association with high blood pressure patients. The AGT levels in serum have been measured by ELISA kit. In this study, Genomic DNA was extracted by DNA extraction kit from both patients and control group and the measurement of concentration and purity was done by the use of nanodrop.  (T-ARMS-PCR) was used to determine the SNPs rs5046 (G/A) in the 5′ near region of AGT gene in hypertension patients and healthy controls, by using specific Primers, The Results detected that genotype CC (34 (68.0) % vs. 50 (100.0) %) was decreased frequency in patients than the control group. In contrast, the CT genotype showed a significant increase (16 (32.0) vs. 0 (0.0) %; OR = 48.30; p<0.001) in the patient group compared with the control group. While the TT genotype does not appear in any groups of patients and controls. The results of the assessment of AGT plasma levels in AGT rs5046 SNP genotypes showed no significant differences according to the rs5046 genotypes in the level of AGT. For CC genotypes, AGT levels were higher in the hypertension patient group than in the control group and significantly (p<0.001). It was concluded investigation indicate that CT was a risk factor for hypertension (EF =31.7).