Detection of ure, emerD and znuA Virulence Genes in Brucella melitensis Isolated from Aborted Fetuses of Sheep in Thi-Qar Governorate

Abstract

Brucellosis is a major and widespread bacterial zoonosis. it is common throughout the world This has serious economic and public health consequences. Sheep, goats, and cows are examples of domestic animals.; suffer significant losses due to Brucellosis. The aim of study was to detect the ure, emerD and znuA virulence genes  in Br. melitensis, samples were  collected from eight fields of sheep in Thi-Qar Governorate south of Iraq during the period November 2022 - to June 2023, from animals that had abortions or certain disease symptoms, , 32 out of 67 mother sheep prove positive results for indirect  Elisa ,32samples of fetal fluids fetus of sheep in Thi-Qar Governorate appeared 32 a positive for the Conventional PCR technique so 32 isolates of Brucella melitensis were determined, Generally. the result of fetal fluids was found  in most prevalent gene was ure, which was found twenty isolates. (68. 75%): while the znuA gene had the lowest proportion of all analyzed genes due to its presence five isolates (15.62%). It was concluded gene were found in certain isolates, such as the emrD gene which found in 14 isolates. (43.01%).