Role of DNA Isolated from Gut Microbiota Escherichia coli in Mice Joints Inflammation

Abstract

The gastrointestinal microbiome is the largest and most diverse reservoir of all the human body niches. The aim of the study relationship between gut microbiota Escherichia coli in mice joints inflammation. In this study, 100 samples of stool were gathered from healthy individual and 20 urine samples from people with recurrent Urinary Tract Infection (UTI) and both types of samples were proceeded accordingly to isolate E. coli strains where 92 strains were isolated from stool samples and 10 from urine samples. The isolated strains were subjected to antibiotic sensitivity test and the results indicated that 39.2% out of the total number of the isolates were multidrug resistance while all the pathogenic strains were multidrug resistant. According to the sensitivity results three isolates were chosen to DNA isolation, two of them isolated from stool samples (Sensitive and Resistant) and one from urine samples. The extracted DNA was divided to two parts one of them was subjected to cleavage by EcoR1 restriction enzyme and the other remains without treatment as a whole DNA, both were injected directly to mice knee joints to study the histopathological effects of bacterial cell free DNA on knee joints. I was conclude that indicated no effect where all the tested tissues were similar to those of group control.