Study Association between DRD2 Gene Expression and Infertility among Some of Iraqi Females

Abstract

Infertility is commonest endocrine disease occurring in women of reproductive age. The aim of  study to determine the D2 dopamine receptors gene expression and clarify altered concentrations of  dopamine in infertile women and controls. Also, to assess their role in infertility and their correlation with measured biochemical parameters .This study was carried out in the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies - University of Baghdad through the period from January 2023- April 2023, The patients were taken from the Kamal Al-Samarrai  Infertility Treatment Hospital in Baghdad the study was included 100 individuals (50 controls and 50 infertile females). Biochemical examinations were done include serum concentrations of dopamine (DA), luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin  were measured by  enzyme-linked immuno sorbent assay  (ELISA) and  enzyme linked fluorescent assay (ELFA)  techniques. The RNA was extracted from whole fresh blood  the concentration and purity of  RNA Based on nanodrop device was performed, then converted to cDNA to measure the level of gene expression for DRD2 by using Real Time PCR technique then converted to cDNA to measure the level of gene expression for DRD2 by using Real Time PCR technique. In this study, the results showed a  significant decreases in dopamine level in infertile Women in comparison to controls group (P< 0.01). In addition The analysis of hormones  level (LH), (FSH) and (PRL) infertile female showed a highly significant increase in PRL and LH compared to controls group (P≤0.01). The results of  the fold expression of DRD2 was showed down-regulated in patient, whereas it was up-regulated in control group. In conclusion gene expression of DRD2 gene and Dopamine  can be utilized as biomarkers for early diagnosis of  female infertility in Iraqi femals as one of the laboratory diagnostic methods.