Molecular Detection of adeL, adeR and adeS Genes of Acintobacter baumannii Isolated from Different Clinical Samples

Abstract

The emergence of multidrug-resistant Acinetobacter baumannii has become a global concern, particularly for patients susceptible to burn, blood bacteremia, and wound infections. In this research endeavor, was collected and analyzed 150 clinical samples from various sites within Baghdad/Iraq hospitals, specifically the Laboratory of Medical City, spanning from October 2022 to March 2023. Through meticulous culturing procedures, A. baumannii isolates were identified via microscopic examination and biochemical assays and Vitek-2 tests. To further validate the identification, a molecular approach was employed, confirming the presence of the 16S-rRNA gene in (n=32) of the A. baumannii isolates using PCR analysis. This molecular verification reinforced outcomes obtained through conventional diagnostic techniques including culture, biochemical tests, chromagar selective media and Vitek-2 tests. An exhaustive antibiotic susceptibility evaluation was performed, revealing multi-drug resistance in 32 isolates. Significantly, all isolates exhibited the presence of the adeL gene, while 31 and 22 isolates carried the adeR and adeS genes respectively. This study was concluded comprehensive insights into the prevalence of multidrug-resistant A. baumannii in Baghdad hospitals. It was concluded  highlighting the significance of molecular techniques in accurate identification and understanding antibiotic resistance patterns.