Study the Expression of miRNA34a-5p Gene among Iraqi Breast Cancer Patients

Abstract

MicroRNAs (miRNAs) are short non-coding RNA sequences (approximately 22 nucleotides) that play an important role in gene regulation. This research is to evaluate the serum expression (miR34a-5p) in the Iraqi population as important biomarkers. Circulating serum miRNA34a-5p expression was measured using RT-qPCR in 50 patients women suffering from breast cancer compared to 50 apparently healthy controls. The significant increases in groups (41-50) years old (p=0.016) and over 60 years old (p=0.02) compared to control groups. 28 years old (46%) of these 50 breast cancer women were under 50 years old, and 22 years old (44%) were over 50 years old. According to body mass index, it is a significant difference in the frequency distribution of patients and control subjects according to BMI (P = 0.020). Where high frequency distribution of patients with breast cancer occurs in the obesity group 23 patients (47%) compared with other groups. The results showed the expression of miR34-5p is high1y significant (p=0.004) in the patients compared with contro1groupe.