Pin1 Gene Expression and Some Biochemical Parameters in Iraqi Population with Chronic Kidney Disease

Abstract

A widespread general medical problem is chronic kidney disease (CKD). It is a primary source of illness and mortality globally and is becoming more acknowledged as a global public health concern, particularly in developing nation. The purpose of this study was to assess the clinical importance of Pin1 gene mRNA expression in samples taken from CKD patients in Iraq and some biochemical parameters. Blood samples from 120 individuals were collected and sorted into the following three groups: Group 1 consists of 40 samples from CKD patients on dialysis, Group 2 of 40 samples from CKD patients who are not on dialysis, and Group 3 of 40 samples from controls who appear to be in good condition. Reverse transcription polymerase chain reaction (RT- PCR) was used to evaluate the messenger RNA (mRNA) expression levels of Pin1 in peripheral blood, and GAPDH was used as a housekeeping gene. Serum parathyroid hormone (PTH) was determined by using ELISA Kit assay. The renal routine test includes serum urea and serum creatinines were detected using an automatic biochemical analyzer (SIMENS ATELLICA, USA). Results revealed that non-Dialysis CKD patients had significantly higher level of Pin1 gene expression (P >0.01) than Dialysis CKD patient and control. The level of serum parathyroid hormone (S.PTH) was show highly significant increase with (p>0.01) in non-dialysis CKD patient than other group (dialysis and control group) while serum urea (S.Uea) and serum creatinin (S.cr) were show high significant increase with (p>0.01) in dialysis group compare to other study groups. It was concluded a high level of expression of Pin1 gene in chronic kidney disease with SHP and high protein level of PTH.