Study the Association of mi 423-5p Expression with Coronavirus-19 Infection in Samples of Iraqi Patients

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent responsible for the extremely contagious sickness known as coronavirus disease 2019 (COVID-19). Non-coding RNAs known as microRNAs (miRNAs) have a vital role in controlling the expression of genes. This study aims to evaluate the role of mi423-5p as a biomarker for the detection and monitoring of COVID-19, it was planned to achieve that through the following steps: a collection of 50 nasopharyngeal swabs and 50 blood samples from the COVID-19 Patient group, and control group (health), detection of gene expression of miR-423-5p by using qRT-PCR.The statistical analysis data of mi 423-5p indicates that there were statistically significant differences between the infected patients and control and patients had the vaccination or not, mi 423-5p was significantly upregulated in patients, that the P value =0.012, and there were significant differences whether the patient had the vaccination or not, the p-value was = 0.028.  The study was concluded that mi423-5p could be useful as a biomarker for COVID-19 infection and it also helps evaluate the effectiveness of vaccines used against COVID-19.