Evaluation of IL10 Gene Expression in Samples of Systemic Lupus Erythematous Iraqi Patients

Abstract

Systemic Lupus Erythematous (SLE) is a type of chronic multisystemic autoimmune disease which impacts women more frequently than males. The antibodies produced by the immune system attack healthy cells The goals of the current research were to evaluate IL10 gene expression in a sample of Iraqi women patients with Systemic Lupus Erythematous and determine the influence of IL10 gene expression level on SLE disease. A total of 54 women blood samples were obtained from individuals diagnosed with Systemic Lupus Erythematous (SLE) in Iraq. Additionally, 46 seemingly healthy women were included as a control group. The age range of all participants was between 15 and 51 years. The assessment of IL10 gene expression was conducted using Real-time Polymerase Chain Reaction (RT-PCR). The results of IL10 gene expression detection showed that no differences between the control group (1 fold change) and the patient group(1.043 fold change). It was  concluded  no effect of IL10 gene expression in development SLE Iraqi patients.