Detection of CTLA4 Gene Polymorphism and Inflammatory Cytokine Profile Among Inflammation Bowel Disease Patients

Abstract

Inflammatory bowel diseases (IBD) are chronic inflammatory conditions of the gastrointestinal tract driven by unsuitable immune responses to an changed gut microbiome in heritably susceptible individuals. The present study was designed to determined the CTLA4 (rs231775) single nucleotide polymorphisms (SNPs)  using sequencing analysis and assessment  the serum levels of interleukin 11,15,18 and CTLA4 level using ELISA technique , that are related to etiology and pathogenesis of inflammation  bowel disease  (IBD) in two groups of Iraqi patients crohn’s disease (CD) and ulcerative colitis (UC).  When comparing IBD patients to controls the  results of CTLA4 (rs231775) SNP revealed some variations, the G allele  was demonstrated increased incidence in IBD patients than controls. The IL11 levels (mean ± SD; pg/mL) were significantly(p≤0.05) increased  (472.61±162.89 and 460.43 ± 144.65) in CD and UC patients, respectively compared with  healthy control (160.15±160.56) pg/ml. Similarly, serum level of IL15 was significantly increased in  crohn’s disease patients and  ulcerative colitis patients (451.18±197.33vs. 395.20 ± 183.54 pg/ml; P > 0.05) , respectively compared to controls (112.15±129.22 pg/ml; p ≤0.05). Whereas, the IL18 levels (mean ± SD; pg/mL) were increased (43.68 ±16.52 and 41.56 ± 10.79) in CD and UC patients, respectively compared with healthy control (40.76 ± 13.27) pg/ml, though, the variation  was not statistically significant. The CTLA4  levels (mean ± SD; pg/mL) were show significantly( p ≤0.05)decreased (0.64 ± 0.28 and 0.61 ± 0.29) in CD and UC patients, respectively compared with  healthy control show a significant (0.80 ± 0.40) pg/ml .It was concluded that CTLA4 SNP effected on CTLA4  protein expression, and cytokines 11,15 were appeared role in IBD than interleukins 18 and these results were indicated  not significantly variation between CD and UC groups (P>0.05).