Gene Expression Analysis of c-Jun and NF-kB (p65) in Gastroduodenal Disorders

Abstract

Gastroduodenal diseases, often associated with Helicobacter pylori (H. pylori) infection, represent significant global health challenges. Understanding the molecular mechanisms and gene expression patterns involved in these conditions is essential for advancing therapeutics. Two key contributors to gastroduodenal disorders are c-Jun and NF-kB (p65), transcription factors that regulate gene expression and inflammation in the gastrointestinal tract. This study delves into the gene expression profiles of c-Jun and NF-kB (p65) in H. pylori-related gastroduodenal disorders. Quantitative PCR was employed to evaluate gene expression in gastric biopsies from 88 participants categorized into four groups based on H. pylori status and gastrointestinal conditions: H. pylori-positive gastritis, peptic ulcers, gastric cancer patients, and H. pylori-negative healthy controls. The comparison in fold change among these groups revealed upregulation of c-Jun and NF-kB (p65) across all patient categories. Notably, gastric cancer patients exhibited the highest fold change in c-Jun (2.49745), while peptic ulcer patients displayed the most substantial increase in NF-kB (p65) (4.87057). It was concluded that c-Jun and NF-kB (p65) signaling pathways play pivotal roles in H. pylori-associated gastroduodenal diseases, offering valuable insights into molecular mechanisms and potential therapeutic targets.