Leptin Gene Polymorphism (rs7799039), Some Serum Hormones and Lipid Profile Parameters in Iraqi Women with PCOS

Abstract

Polycystic ovary syndrome (PCOS) is a complicated, heterogeneous disorder, which affects 6–20% of women of reproductive age, causes a range of problems in the reproductive, metabolic, and endocrine systems. Polycystic ovaries, hyperandrogenism, and prolonged anovulation are its defining features. It appears differently as a result of a number of interconnected factors, including heredity, dietary habits, and environmental exposure. This study aimed to study of leptin gene polymorphism rs7799039 and some serum parameters in Iraqi women with polycystic ovary syndrome. By using ELISA kits, the levels of leptin and the soluble leptin receptor (sOB-R) were measured. Except for anti-mullerian hormone (AMH), which was assessed by Elisa, and the lipid profile parameters, which were measured by spin 200 full auto, other hormones were measured by cobas E411. HRM-PCR was used to determine the  genotypes and allele frequency of rs7799039 SNP at Leptin gene. PCOS patients had significantly (p˂0.05) higher serum leptin and sOB-R levels than controls (4303.28±71.55 versus 3854.84 ±213.96 pg /ml and 144.09 ±5.51 versus 122.16 ±7.64 ng/ml, respectively). The frequency of GG genotype was in PCOS patients significantly lower than in apparently healthy subjects (18.3 % versus 60%, respectively),while  the frequencies of GA and AA genotypes were significantly greater in PCOS patients compared to controls that appear to be healthy (56.7% versus 36.6% and 25.0% versus 3.4%, respectively).The G allele frequency was higher in apparently healthy subjects than in PCOS women (78.3% versus 46.6%, respectively) while A allele frequency was significantly greater in PCOS patients compared to people who appear to be healthy (53.4% vas 21.7%, respectively). It was concluded an A allele-related risk factor for PCOS in iraqi patients. Sgnificant difference between GA and AA genotypes in estrogen hormone and significant difference between  GG,GA and AA genotypes in AMH hormone were noted.