Genetic Polymorphism of IL-6 and TLR-4 Genes Correlated with E.coli Infection in UTI and Diabetic Patients

Abstract

Urinary tract infections (UTIs) and diabetes have a complex relationship that works both ways. UTIs are more common in people with diabetes mellitus (DM). The aim of the study a potential role of TLR-4 in the dynamics between diabetes and UTIs . Blood samples were collected from 100 patients with UTI and 100 patients with DM patients groups in addition to 100 apparently in individual (control group). The IL-6 and TLR-4 genes were amplified upon DNA extraction and submitted for sequencing. In addition, bacterial DNA has been extracted and the specified DNA sequence of the PapA gene was amplified. The results of rs1800797 showed higher frequency of the AA and AG genotypes within the patients than control group. The GG genotype showed a higher frequency in control group than patients group. The genotype frequencies of the SNP showed a none-significant higher frequency of the GG in control group than patient group. The genotype CG showed a non-significant higher frequency in patients (27.27) than in control (10). The genotype GG showed a significant (P-Value=0.0065) higher frequency in control (80) than in patient (19.09) with a high protection value (odd=0.0278). The genotype AA showed a none-significant (P-Value= 0.5383) higher frequency in control groups (90) than in control (72.72) with high-risk ratio (odds=0.444). The genotype AG showed a non-significant higher frequency in patients (18.18) than in control (10) (odd= 2.25, P-Value= 0.5383 and C.I.= 0.1701 to 29.7687). 25% of samples were positive for E. coli, underscoring a notable prevalence of this bacterium within the group under investigation. Urinary tract infections (UTIs) and diabetes have a complex relationship that works both ways. UTIs are more common in people with diabetes mellitus (DM). Blood samples were collected from 100 patients with UTI and 100 patients with DM in addition to 100 apparently healthy control group. The findings revealed that the expression levels of IL-6, TLR-4, higher in individuals with both diabetes and UTI than in those with diabetes. There was a Investigations into the relationship between IL-6 (TLR-4) inflammation and immune system regulation have shown that IL-6 can control the immune response by either upregulating or downregulating