Detection of NLRP3 Gene Polymorphism and Evolution of Some Biomarkers in SARS-CoV-2 Patient

Abstract

The polymorphisms in the genes of NLR family pyrin domain containing protein 3 (NLRP3) and inflammatory markers are related to the severity and Susceptibility of coronavirus 2019 (COVID-19). The aim of the study to determine the role of variants in the genes of NLRP3 and their effects on severity and/ or susceptibility in COVID-19 disease we used allele-specific primer method to analyze NLRP3 variants rs35829419, rs10754558 also, The infection with COVID-19 in the first stage leads to Neutrophilia, while the increased severity of the disease leads to lymphopenia, and the first indicator for storms of cytokine is the neutrophil-to-lymphocyte ratio (NLR). Therefore, the current study is designed to offer data for local information about parameters of hematology among patients with COVID-19 and evaluate their relationship with further factors. Between December and October, 2022 blood specimens and nasopharyngeal swabs (99) were taken from patients with COVID-19. Samples were established by antibody IgM and IgG for COVID-19, real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assay, as well as analysis of blood samples to test the hematological parameters. The results exhibited NLPR3 rs35829419 A allele and genotype CA are related to susceptibility and the presence of COVID-19. NLR, CRP, D-dimer, Ferritin, LDH were increased significantly and associated with the disease severity. It was concluded the NLR can be used as a suitable biomarker assistant to laboratory records in the progression of disease and evaluating the severity of the cases.