Biomedical Applications of Bacteriocin Purified from Lacticaseibacillus paracasei as Antimicrobial and Antibiofilm Compound against Pathogenic Bacteria and Yeast

Abstract

Gastrointestinal systemic infections caused by bacteria and fungus as well recognized to be the main reason for causing acute gastroenteritis; Bacteriocins have a wide spectrum of antibacterial and antifungal efficiency. Hence the present experiment was designed to examine the antibacterial, antifungal and antibiofilm eligibility of Bacteriocin over locally isolated pathogens including Staphylococcus aureus, Escherichia coli and Candida albicans. Bacteriocin was purified from local strain of Lacticaseibacillus paracasei that identified by the 16S rRNA gene, the identification of Candida albicans isolate was confirmed by ITS gene. The antimicrobial activity was examined by well diffusion assay and MIC method by the concentration 0.54 mg/ml for Bacteriocin. Meanwhile, antibiofilm dispersal consideration have been completed by Microtiter plate method. The outcomes revealed to highest inhibition observed over S. aureus isolates ranged from 23.5 mm to 39 mm, the minimum inhibitory concentrations were 0.05 mg/ml for all pathogenic indicators, while biofilm suppression show high reduction in biofilm against C. albicans with OD (0.13). In conclusion, Bacteriocin has obvious antibacterial, antifungal effect and biofilm dispersal ability and could be used towards Gram- positive, Gram- negative and yeast.