The rs233569 Polymorphism of the ACE2 Gene in Iraqi Hospitalization Patients with SARS-COV2

Abstract

During the year 2020, the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV2), inflicted considerable damage and devastation upon the global populace, resulting in the unfortunate demise of approximately 6.5 million individuals .This study was conducted from January 2022 till August 2022  on 70 hospitalized patients infected with COVID-19 in Dar Al Salam Field  Hospital and Dar Al Shifa Hospital affiliated to the Medical City with age  range of 19-60 years and 70 healthy subjects with the same age . Blood samples were collected from all participated for DNA extraction and detection the rs233569 polymorphism of the ACE2 gene using Tetra Arms Technique. Primers were designed for the tetra amplification refractory mutation system polymerase chain reaction technique (tetra ARMS PCR) based on their corresponding sequence in the National Center for Biotechnology Information NCBI https://www.ncbi.nlm.nih.govpmc. The results of rs233569 genotype revealed three genotypes (CC, CT, TT) frequency distribution in both SARS- COV2 patients and controls the homozygous genotype (TT) appeared with a significant increase (0≤ 0.05) in SARS- COV2 patients compared with control. The homozygous genotype (CT) had also appeared with highly significant increase (0≤ 0.01) in SARS- COV2patients compared with control.  Resulting that heterozygote CT was a higher risk of SARS- COV2 patients comparing with genotype TT that estimated a significant increase. Allele C allele is more frequent in both in SARS- COV2 patients and control.