The Prevalence and Sequencing of the Pathogenic Genotoxic Gene (clbA) in Bacteria E.coli from Patients with Bladder Cancer and UTI in Baghdad Province

Abstract

Bladder cancer is among the most prevalent cancers worldwide, with 549,393 new cases reported in 2018. Approximately 3% of all new cancer was diagnoses and 2.1% of all cancer deaths are due to urinary bladder cancer. Carcinoma of the urinary bladder is one of the most prevalent cancers in Iraq. This study aims to collect 50 blood and urine samples from patients suffering from urinary tract infections and bladder cancer, and 50 urine and blood samples from healthy people considered to be control, with age ranged from 30-85 years old, from different hospitals (ALyarmouk hospital, Medicine city and Royal hospital) in Baghdad/Iraq between the period from October 2022 until August 2023. Our study is intended for most likely the first time in an Iraqi series, study the relationship between E.coli bacteria and its gene colibactin with bladder cancer. The isolation and identification of bacteria E.coli from patients by traditional and molecular methods was done, and an estimate of the virulence gene (clbA), the result found that (4 samples) 8% of the 50 patients case were having the colibactin gene. And finally nucleotide sequencing of the PCR products of the clbA gene has been performed for 4 positive clbA gene samples.