Evaluation Antibacterial Activity of Quercetin Against XDR - Pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa is an opportunistic bacteria  that is commonly linked to nosocomial infections and demonstrates resistance to many antibiotics. Thus, many groups of secondary metabolites from plants have been used in a number of investigations conducted in the past few years. According to reports, quercetin possesses pharmacological properties that include antibacterial and antioxidant, actions. The aim of the research was to study antoxidant and antibacterial activity of quercetin against XDR-resist pseudomonas aeruginosa.  isolates of pseudomonas aeruginosa   from different clinical sources was collected , laboratory diagnosis include morphological and biochemical testing, confirmed with  molecular identification by 16rRNA detection. Susceptibility test was applied to detect bacterial resistance to druge. The total phenolic content and antioxidant activity of quercetin was determined using the Folin-Ciocalteu method, and DPPH assay. The antibacterial activity against pseudomonas. aeruginosa was measured by determining MIC value using microtiter plate method. The results of this study shows that from 53 isolates. Ten (19%) of the bacterial isolates are resistant to more than five antibiotic classes, which are referred to as XDR. Total phenolic content  and antioxidant activity of quercetin  demonstrating that quercetin was superior than the synthetic antioxidant, and was  rich in phenolic compound ,the antibacterial activity  against pseudomonas. aeruginosa  It was conducted that No impact of quercetin on growth of XDR-pseudomonas aeruginosa.