Efficacy of Curcumin Selenium Nanoparticles to Target GDF9 Gene and Redox Status in Doxorubicin Stressed Rats

Abstract

Background Curcumin extract contain many bioactive polyphenolic compound, such as curcuminoids, dimethoxy-curcumin  and bisdemethoxycurcumin obtained from curcuma longa  which exhibits a positive effect on female reproductive system.Current study was aimed to investigate the  protective effect of synthesized curcumin selenium nanoparticles (CurSeNPs) utilizing hydroalcoholic extract derived from Curcuma longa (turmeric) in alleviating the disturbance of redox homeostasis and GDF9 gene expressions related to ovarian functions doxorubicin treated in female rats. CurSeNPs were synthesized by combining sodium selenite with an alcoholic extract of Curcuma longa, resulting in a color shift of the solution from light orange to orange-red after 24 hours. The UV spectroscopy data indicated absorption bands at a wavelength of 214 nm, FTIR analysis demonstrated that CurSeNPs compounds contain abundant OH, C-H, C=O, C=C, C-C, and amine groups. Additionally, X-RD analysis at 2 theta identified the formation type as curcumin and indicated the particle size to be within the range of 18–80 nm and EDX analysis has verified the existence of elemental selenium nanoparticles exhibited excellent stability, as evidenced by their high zeta potential. Besides, the FESEM picture demonstrated the existence of spherical CurSe nanoparticles within the size range of 21–37 nm, as confirmed by AFM.  In vivo study, included thirty-two (32) adult female rats were randomly and evenly divided into four experimental groups and treated accordingly for a duration of two weeks as following: Control (C) group: Rats were administered orally distilled water, group G1: Rats were (IP) injection of Dox (4.40mg /kg B.W.), (G2) group: Rats were administered CurSeNPs (10.47 μg /kg B.W.) and (G3) group: Rats were subjected to both Doxorubicin and intubation with CurSeNPs at same doses. Blood samples were collected after 14 days of the experiment from anesthetized rats, then serum were obtained for measuring SOD and MDA. Furthermore, the specimens of ovaries tissues were extracted to analyze the gene expression of GDF9. The results pointed the creation of oxidative stress in the G1 group, characterized by a significant reduction in serum SOD concentration and GDF9 gene expression level associated with an increase in serum MDA levels. In contrast, the present trial showed that the administration of CurSeNPs in G2 and G3 groups has ability to the improvement the oxidative stress-related factors as well as increase the express of GDF9gene. It was concluded the current study demonstrated that CurSeNPs have both preventive and /or therapeutic effects against doxorubicin toxicity in adult female rats may be via as antioxidant and anticancer properties.