Investigate Prevalence of (blaIMP, blaOXA-40, and blaGES) Genes in Carbapenems Resistance Pseudomonas aeruginosa Isolates

Abstract

Pseudomonas aeruginosa, a Gram-negative bacterium, is renowned for its versatility and adaptability, Morphology and Structure of P. aeruginosa is a rod-shaped bacterium, typically 0.5 to 3 microns in length, with a polar flagellum for movement. Is to determine resistance to antibiotics, especially carbapenems, and to determine the presence of the genes blaIMP, blaOXA-40 and blaGES in Pseudomonas aeruginosa bacteria, Multidrug-resistant Pseudomonas aeruginosa poses a global concern, particularly for individuals susceptible to burns, blood bacteremia, and wound infections. This study aimed to gather and analyze 150 clinical samples from various Baghdad hospitals, detecting and confirming Pseudomonas aeruginosa isolates, evaluating antibiotic susceptibility, exploring resistance-linked genes, and estimating the presence and prevalence in Pseudomonas aeruginosa isolates meropenem. Samples underwent examination at the University of Baghdad, Institute of Genetic Engineering and Biotechnology. Identification methods included chemical tests, microscopy, cetrimide agar, VITEK-2 system, and 16S-rRNA analysis. Conventional PCR identified target genes (blaIMP, blaOXA-40, blaGES). Among 150 samples, 38 were P. aeruginosa isolates. Molecular analysis revealed blaIMP absence, with blaOXA-40 and blaGES presence in 20 and 18 isolates, respectively. It was concluded that target genes likely contribute to meropenem resistance.