Prevelance of Stn and FimH Genes in Salmonella enterica Isolated from Patients in Thi-Qar Governorate

Abstract

Salmonella spp.  are a significant cause of diarrheal illnesses worldwide and are among the most hazardous bacteria transmitted through food. The aime of study detect the existence of crucial virulence genes in Salmonella enterica (S. enterica) bacteria obtained from stool samples gathered from Thi-Qar hospitals in Iraq. A total of 100 stool samples were obtained from patients with diarrhea symptom  at several hospitals in Thi-Qar. The isolates were initially identified through morphological  characteristic on XLD media and later confirmed using the Vitek-2 method. The DNA was extracted from (20) isolates using a genomic DNA kit. The PCR technique was utilized for detection of Salmonella enterica  by using invA .Other virulence genes suvh as FimH, and Stn at the molecular level. Out of the 100 swab samples , 20% (20 samples) were positively identified as S. enterica. The occurrence of invA, Stn and FimH virulence genes was percentage (100%) and in (75%) of the samples (15 out of 20) and (20%) of the samples (4 out of 20 ), respectively . The available findings confirm the presence of Salmonella enterica. with a wide array of virulence genes in stool samples, thus establishing these bacteria as a substantial public health risk.