Investigate some Biochemical Parameters and Impact of NOS3 Gene Polymorphism among Iraqi Patients with Chronic Kidney Disease

Abstract

Chronic kidney disease CKD as a kidney damage with persistence about three months or longer, as well as it represents an especially large load in low- and middle-income countries, which are less equipped to deal with its consequences. Chronic kidney disease has revealed as one of the leading causes of mortality worldwide. The aim of the current study was to investigate the association between genetic polymorphism of NOS3 genes and the severity of CKD in Iraqi population. Fifty Iraqi CKD patients (males 17 and females 33) and fifty apparently healthy as control group (male27 and females23) aged 20 –65years old were involved in the study. They were diagnosed by specialist physicians and estimation GFR<15 were chosen as CKD patients. The result of the current study according to the demographical distribution gave different outcomes, in relation to age were significant the highest percentage (34%)of the disease appeared and it was diagnosed at age 50-59 years old. The sex result show no significant differences between the two studied groups. According to the clinical aspect of diagnosis of patient were depended on several   criterial basically it is measuring the level of serum creatinine and urea blood the result was significant (P≤0.05) in CKD patients 12.43±6.20and 136.26±38.80 respectively, Versus the control group 0.64±0.11, 20.58±4.55. The results showed a significant decrease in both hemoglobin and iron levels in CKD patients 9.00±1.84, 10.17±7.24 respectively, Versus the control group 13.12±1.75, 18.80±3.97. More over according to ferritin shows a significant increase in the serum level for CKD patients 506.54±86.16 Versus the control group 126.18±72.56 and according to hypertension, whereas 92% of the CKD patients are hypertension,with respect to NOS3 gene polymorphisms, allele and genotype frequencies showed significant differences between CKD patients and apparently healthy individuals. The Nos3 gene located in intron on chromosome 7, it involves a polymorphism (C to T, rs 2070744). The study found that carriers with the TT and CT genotype showed statistically significant differences at a significant level of p≤0.05 and OR more than 1. As a conclusion of the current study the effect of the variant allele for SNPs (rs 2070744C>T) of NOS3 gene appears to be related with chronic kidney disease, that suggests to have a modifier effect on the disease incidence.