Molecular Detection of Some Virulence Genes in K. pneumonia Coinfection with COVID -19 Iraq Infection

Abstract

The coronavirus pandemic of 2020 was caused by the coronavirus disease 2019 (COVID-19), an infectious disease with severe acute respiratory syndrome that was first discovered in Wuhan, China. Since then, it has spread throughout the world. Co-infections between viruses and bacteria are among the deadliest medical conditions, with greater fatality rate. Research on bacterial superinfections in patients with coronavirus disease 2019 (COVID-19) is scarce. COVID-19 patients are at risk for colonization with K. pneumonia and hospital-acquired infections. Therefore, Infections with K. pneumonia, particularly those caused by extremely virulent strains, may make COVID-19 more difficult to treat. Fifty sample of COVID-19 infection collected and detected positive by (RT) PCR with 50 apparently control healthy sample than we cultured  50 sputum and nasal pharyngeal  swabs for all COVID19 positive samples ,fifty sample of coinfection bacterial in the COVID19 patients identified by A- Vietk-2system ,B-confirmed by molecular detection PCR  , The fifty-isolate distributed as 27 (54 %) k. pneumonia, 6 (12 %) E. coli, P. aeruginosa 15 (30%), other bacteria 2(4%)the k. pneumonia is the largest number of coinfection . The results of the current study demonstrated a positive correlation between the presence of certain virulence genes and the ability of bacterial isolates to resist antibiotics. Certain virulence genes (rmpA, mrkA, and iuCc, ybts, magA,) have been found by molecular means.  our study was done by conventional PCR technique. we found Klebsiella pneumonia isolates which was found rmpA18(66%) of this isolates at bp 535, then 7(25%)) isolate were positive for mrkA gene at 115bp,whereas iucC gene was recorded in 15(55%) isolates at bp 1075 , magA found in  3 (11%) at 1283,  and ybts found in 20(74%) at 242 bp.