Toll-Like Receptor 9 Serum Level and Genetic Polymorphism Linked with Type Ii Diabetes Mellitus Among Iraqi Patients

Abstract

The rising incidence of Type 2 diabetes mellitus (T2DM) globally attaining epidemic levels and is emerging as a significant public health concern. Toll-like receptors (TLRs) are innate immune system receptors that facilitate the inflammation  in diabetic  mellitus disorder. The study evaluates the relationship between rs352139 SNP in TLR9 gene in patients with T2DM in Iraq, and its effect on TLR9 level in those patients. This study included 60 T2DM cases and 40 apparently healthy controls. The SNP were identified using Sanger sequencing for rs352139. Current findings indicated a significant elevation in TLR9 levels in T2DM patients compared to healthy people (1.182± 0.76 versus 0.836 ± 0.74 ng /ml, p ≤ 0.05). T2DM risk factors include AA, GG genotypes in comparison with controls (40% versus 22.5%; P = 0.009; OR = 2.30 and 40% versus 20%; p = 0.005; OR 2.67). Patients carrying the AG and GG genotypes, had higher Serum concentrations of TLR9 in comparison with the control group (1.13 ± 0.26 versus 0.79 ± 0.18 ng/ml; 1.22 ± 0.18 versus 0.71 ± 0.16 ng/ml) respectively. This study indicate a risk association between rs352139 and T2DM. Also, rs352139 increase TLR9 serum level in patients with T2DM.