Global Registration of Environmental Bacteria Recorded in NCBI, ENA and DDBJ for New Iraqi Isolates

Abstract

The current study included the identification and new global registration of bacteria isolated from two environmental sources (soil and water). The Goal of this research is screening the microbiome community with a highly adaptability of new bacterial strains grown in environment. The molecular identification of 28 isolates  was successfully performed using the amplification and sequencing of the 16SrRNA gene. Additionally, bacterial identification was confirmed through Gram staining. Fifteen samples isolated from soil and Thirteen from water were recorded as new strains in the National Center for Biotechnology Information (NCBI) and the Gene bank Data. Results showed, that the bacterial species involved:  (6) Bacillus subtiles, (1) Psychrobacter nivimaris, (2) Terribacillus aidingensis, (1) Zobellella denitrificans, (1) Bacillus cabrialesii, (2) Staphylococcus gallinarum , (1) Photobacterium halotolerans, (1) Bacillus paramycoides, (1) Pseudomonas putida,    (4) Staphylococcus epidermidis, (3) Staphylococcus haemolyticus,  (1) Rheinheimera aquimaris, (1)Planomicrobium okeanokoites,, (1)Micrococcus endophyticus,  (1) Rheinheimera  baltica, and (1) Staphylococcus saprophyticus. Gene investigations were performed on all isolates and the phylogenetic tree of the isolates was constructed using MEGA 11 software. These strains identified based on phenotypic properties and molecular techniques. The new bacterial strain exhibited a significant performance in environment.