Cytotoxicity of 1, 2-Dihydroxybenzene Towards Hep-2 and AMGM5 Cancer Cell Lines in Presence of SuperOxidase Dismutase and Peroxidase

Abstract

The cytotoxic effect of 1,2-Dihydroxybenzene(catechol) was studied in human cancer cell lines, Epidermoid larynx carcinoma  (Hep-2) and Cerebral glioblastoma multiforme (AMGM5), catechol undergo autoxidizes in physiological buffer to quinones. The results showed that catechol has a fatal effect to these cells after 72h of exposure .This toxicity was connected to the creation of quinones. There was a noticeable defeat of cell viability in a dose reliant manner in both cell type. Cytotoxicity was vetoed by the adding of 100ul SOD, while the addion of 500 ul POD or SOD combined with POD did not upturn the inhibition encouraged by SOD alone in both cells type.